Bauhinia purpurea Lectin (BPL/BPA) - HRP (Horseradish Peroxidase)

Bauhinia purpurea Lectin (BPL/BPA) - HRP (Horseradish Peroxidase)
Bauhinia purpurea (Camel's foot tree) Lectin (BPL/BPA) - HRP (Horseradish Peroxidase)
Product image 1Bauhinia purpurea Lectin (BPL/BPA) - HRP (Horseradish Peroxidase)
Product image 2Bauhinia purpurea (Camel's foot tree) Lectin (BPL/BPA) - HRP (Horseradish Peroxidase)

Price: Regular price $176.27

SKU#: 21510924-1

Product Description

Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 195,000. Binding appears to be highest for glycoconjugates containing galactosyl (β-1,3)N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates.

Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct. Makela's group 2 sugars, particularly N-acetyl-D-galactosamine, are potent inhibitors. The native protein appears to be stable in detergent solution.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Bauhinia purpurea Lectin (BPL/BPA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Technical Specifications

Abbreviation: BPL/BPA
Material Source:: Camel's foot tree
Conjugate:: Horseradish Peroxidase (HRP)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galβ3GalNAc
Inhibiting or Eluting Sugar:: Lactose
Divalent Ions:: None Required
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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