Arachis hypogaea Lectin (PNA) - HRP (Horseradish Peroxidase)

Product Description

Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. Galactose and lactose are poor inhibitors while disaccharide Gal ß1-3GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. Although extracts of Arachis hypogaea do not agglutinate untreated or trypsin-treated human erythrocytes, PNA has been known to agglutinate neuraminidase-treated human red blood cells. PNA may be used for clinical determination of T-polyagglutinability of erythrocytes and probing cell membranes of normal and malignant cells. This Lectin can be of use in characterization of tumor-specific antigens on the surface of malignant cells. It can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Arachis hypogaea Lectin is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Recommended Usage: Recommended dilutions 0.5 – 10 µg / ml in 20 mM Tris buffer pH 7.4 containing 1mM CaCl2 and 0.5 mM MnCl2, 50 mM NaCl. Less than 1 µg/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.