Allium sativum Lectin (ASA) - HRP (Horseradish Peroxidase)
Price: Regular price $222.53
Product Description
Allium sativum lectin (ASA) is isolated from garlic and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity. ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research.
Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Allium sativum Lectin (ASA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.
Application: Immunohistochemistry, Immunocytochemistry, Western Blotting, Glycobiology.
Recommended Usage: Recommended dilution ranges 0.5 – 10 µg/ml. 1XPBS can be used for reconstitution. Less than 0.5 ug /ml will agglutinate rabbit erythrocytes.
Technical Specifications
| Abbreviation | : | ASA |
| Material Source: | : | Garlic |
| Conjugate: | : | Horseradish Peroxidase (HRP) |
| Concentration: | : | 2 mg/mL |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Mannose |
| Inhibiting or Eluting Sugar: | : | α(1,3)-Linked Mannosyl Units |
| Divalent Ions: | : | None Required |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
