Artocarpus integrifolia Lectin (AIA) - HRP (Horseradish Peroxidase)

Artocarpus integrifolia Lectin (AIA) - HRP (Horseradish Peroxidase)

Price: Regular price $185.01

SKU#: 21510025-1

Product Description

Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography (SKU: 21510001) and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein's structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal and 2-deoxy-α-D-galactose. Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods.

Specifications:

  • Source: Artocarpus integrifolia (Jackfruit)
  • Activity: Less than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes.
  • Protein Concentration (Based on OD 280): 1 mg purified AIA-HRP / 1 mL buffer
  • Carbohydrate Specificity: Galß1-3GalNAc
  • Inhibitory Carbohydrate: Galactose or Melibiose
  • Mitogenic Activity: Yes
  • Divalent ions required: None
  • Conjugation: HRP

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.

Technical Specifications

Abbreviation: AIA
Material Source:: Jacalin
Conjugate:: Horseradish Peroxidase (HRP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galβ3GalNAc
Inhibiting or Eluting Sugar:: Galactose or Melibiose
Divalent Ions:: None Required
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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