Amaranthus caudatus Lectin (ACL/ACA) - HRP (Horseradish Peroxidase)
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Product Description
Affinity-purified Amaranthus caudatus (ACL) (SKU: 21510158) is a dimer glycoprotein made up of two subunits. ACL has preferential specificity to oligosaccharides containing the galactosyl disaccharide Galβ3GalNAc. ACL tolerates sialic acid substitution at the 3 positions of galactose in the “T” antigen. It does not have specificity for blood groups but does have a higher affinity for type O erythrocytes over B and A.
Horseradish peroxidase (HRP) is a 40 kDa enzyme that catalyzes oxidation reactions, producing a colored or fluorescent product, commonly used in blotting, immunoassays, and immunohistochemistry. Amaranthus caudatus Lectin (ACL/ACA) is conjugated to HRP in an optimized ratio to ensure optimal staining characteristics for various applications.
Recommended Usage: Recommended dilutions 0.5 – 10 µg/ml in 1XPBS. Less than 1 µg of ACL will agglutinate human, sheep, goat, rat, rabbit and guinea-pig erythrocytes.
Technical Specifications
| Abbreviation | : | ACL/ACA |
| Material Source: | : | Tassle flower |
| Conjugate: | : | Horseradish Peroxidase (HRP) |
| Concentration: | : | 2 mg/mL |
| Molecular Weight: | : | 54 kDa |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galβ3GalNAc |
| Inhibiting or Eluting Sugar: | : | Disialyzed Fetuin |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Western blotting, Cell typing, Glycobiology.
References
- Rinderle SJ, Goldstein IJ, Matta KL, Ratcliffe RM. Isolation and characterization of amaranthin, a lectin present in the seeds of Amaranthus caudatus, that recognizes the T- (or cryptic T)-antigen. J Biol Chem. 1989 Sep 25;264(27):16123-31.
- Rinderle SJ, Goldstein IJ, Remsen EE. Physicochemical properties of amaranthin, the lectin from Amaranthus caudatus seeds. Biochemistry. 1990 Nov 20;29(46):10555-61.
