Ulex europaeus Lectin (UEA II) - Cy3

Ulex europaeus Lectin (UEA II) - Cy3

Price: Regular price $178.12

SKU#: 21511207-1

Product Description

Affinity purified Ulex europaeus agglutinin (UEA II) is isolated from gorse seeds and separated from crude extract. This lectin is a glycoprotein that consists of four 24,000 Da monomer subunits that require Ca2+ for binding to its ligands through carbohydrate recognition domain. It is specific for di-N-acetylchitobiose, an oligomer of GlcNAc. The monosaccharide GlcNAc is not an inhibitor of the lectin activity. UEA II has no mitogenic activity and agglutinates type O erythrocytes with greater interest than type A3 erythrocytes. Addition of EDTA eliminates agglutinating activity. Agglutinating activity can be recovered with the addition of calcium.UEA II is a potent inhibitor of the lectin complement pathway following endothelial oxidative stress. The molecular mechanism of UEA II-induced inhibition of the lectin complement pathway appears to be through competition for a common binding site on the endothelial cell and not at the level of mannose-binding lectin (MBL) inhibition. See other UEA II conjugates.Cy3 can be used to visualize the binding pattern of Ulex europaeus Lectin (UEA II) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: UEA II
Material Source:: Gorse
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Oligomers of β(1,4) - linked N-Acetylglucosamine
Inhibiting or Eluting Sugar:: GlcNAc β(1,4) GlcNAc
Fluorescence:: Orange
Divalent Ions:: Ca++, Mn++, Zn++
Excitation:: 550 nm
Emission:: 570 nm
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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