Allium sativum Lectin (ASA) - Cy3
Price: Regular price $169.74
Product Description
Allium sativum lectin (ASA) is isolated from garlic and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to several α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity. ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research. Cy3 can be used to visualize the binding pattern of ASA in cellular imaging and flow cytometry.
Cy3-labeled ASA is ideal for visualizing binding patterns in cellular imaging and flow cytometry. This dye offers superior photostability compared to many other fluorophores and is compatible with TRITC filter sets. Cy3 is commonly paired with green-fluorescent dyes for effective dual-labeling.
Applications: Immunocytochemistry, Immunofluorescence, Anti cancerous, Glycobiology
Recommended usage: Recommended dilution ranges 0.5 – 10 µg/ml. 1XPBS can be used for reconstitution. Less than 0.5 ug /ml will agglutinate rabbit erythrocytes.
Technical Specifications
| Abbreviation | : | ASA |
| Material Source: | : | Garlic |
| Conjugate: | : | Cyanine 3 (Cy3) |
| Concentration: | : | 1 mg/mL |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Mannose |
| Inhibiting or Eluting Sugar: | : | α(1,3)-Linked Mannosyl Units |
| Fluorescence: | : | Orange |
| Divalent Ions: | : | None Required |
| Excitation: | : | 550 nm |
| Emission: | : | 570 nm |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
