Ulex europaeus agglutinin (UEA I+II) is affinity purified consists of two hemagglutinins (UEA I and UEA II). The two lectins can be separated by salt or ethanol precipitation. Both have been sequenced and are homologous with each other, as well as homologous with other legume lectins of various specificities. Carbohydrate-binding peptides have been isolated for each lectin after peptidase digestion, and these peptides also exhibit homology with other such peptides. UEA I interacts specifically with blood group H type 2 antigen, which is expressed in individuals with type O blood and to a lesser degree in individuals with type A or B blood. UEA II is not blood group specific but the best inhibitors are the H type oligosaccharides.Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Ulex europaeus Lectin (UEA I+II) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.