Succinylated Triticum vulgaris Lectin (Succ WGA) - Cy3
Price: Regular price $153.32
Product Description
Succinylated wheat germ agglutinin (Succ-WGA) is affinity purified from the native wheat germ agglutinin (WGA). It is dimeric at pH as low as 5 and dissociates at pH lower than 4.5. Succ-WGA agglutinates rabbit erythrocytes at a minimal concentration of about 2g/ml, and agglutinates human erythrocytes of blood groups A, B, and O types. The solubility of Succ-WGA is around 100 times higher than the unmodified lectin at neutral pH. Ulike native WGA, Succ-WGA does not bind to sialic acid residues but retains its specificity towards N-acetylglucosamine. The use of conjugates of the native WGA and the succinylated allows to distinguish between sialylated glycoconjugates and others N-acetylglucosamine structures. See other Succ-WGA conjugates.
Cy3 can be used to visualize the binding pattern of Succinylated Triticum vulgaris Lectin (Succ WGA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.
Technical Specifications
| Abbreviation | : | Succ WGA |
| Material Source: | : | Wheat |
| Conjugate: | : | Cyanine 3 (Cy3) |
| Concentration: | : | 1 mg/mL |
| Molecular Weight: | : | N/A |
| Molecular Formula: | : | N/A |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | N-Acetylglucosamine |
| Inhibiting or Eluting Sugar: | : | Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt |
| Fluorescence: | : | Orange |
| Divalent Ions: | : | Ca++ |
| Excitation: | : | 550 nm |
| Emission: | : | 570 nm |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
