Succinylated Triticum vulgaris Lectin (Succ WGA) - Cy3

Succinylated Triticum vulgaris Lectin (Succ WGA) - Cy3
Succinylated Triticum vulgaris (Wheat) Lectin (Succ WGA) - Cy3
Product image 1Succinylated Triticum vulgaris Lectin (Succ WGA) - Cy3
Product image 2Succinylated Triticum vulgaris (Wheat) Lectin (Succ WGA) - Cy3

Price: Regular price $153.32

SKU#: 21761146-1

Product Description

Succinylated wheat germ agglutinin (Succ-WGA) is affinity purified from the native wheat germ agglutinin (WGA). It is dimeric at pH as low as 5 and dissociates at pH lower than 4.5. Succ-WGA agglutinates rabbit erythrocytes at a minimal concentration of about 2g/ml, and agglutinates human erythrocytes of blood groups A, B, and O types. The solubility of Succ-WGA is around 100 times higher than the unmodified lectin at neutral pH. Ulike native WGA, Succ-WGA does not bind to sialic acid residues but retains its specificity towards N-acetylglucosamine. The use of conjugates of the native WGA and the succinylated allows to distinguish between sialylated glycoconjugates and others N-acetylglucosamine structures. See other Succ-WGA conjugates.

Cy3 can be used to visualize the binding pattern of Succinylated Triticum vulgaris Lectin (Succ WGA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: Succ WGA
Material Source:: Wheat
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Molecular Weight:: N/A
Molecular Formula:: N/A
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylglucosamine
Inhibiting or Eluting Sugar:: Chitin Hydrolysate or N-Acetylglucosamine w/acid or salt
Fluorescence:: Orange
Divalent Ions:: Ca++
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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