Sambucus nigra Lectin (SNA/EBL I+II) - Cy3
Price: Regular price $201.56
Product Description
Affinity purified Sambucus nigra lectin (EBL I+II, also known as SNA) is is composed of two ricin-related lectins. EBL I+II is a tetrameric glycoprotein with a recognition of the Neu5Ac(α2-6)Gal or GalNAc linkage in glycoconjugates.EBL I+II is not blood group specific but it does agglutinate type A erythrocytes more strongly than either B or O cells. Trypsin treated erythrocytes react better than untreated cells. Neuraminidase treated erythrocytes react with this lectin due to the presence of terminal galactose resides. SNA has a broad specificity for both α- and β-linked galactose, as well as unrelated sugars such as fucose. It has been determined that the lectin does not bind to glycoproteins or glycolipids containing only terminal α(2,3)-linked sialic acid residues.EBL I is composed of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. EBL II consists only of carbohydrate-binding B-chains. EBL I+II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems independent from the catalytic activity of the A-chain, but depends on the carbohydrate binding B-chain.Cy3 can be used to visualize the binding pattern of Sambucus nigra Lectin (SNA/EBL I+II) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.
Technical Specifications
Abbreviation | : | SNA/EBL I+II |
Material Source: | : | Elderberry |
Conjugate: | : | Cyanine 3 (Cy3) |
Concentration: | : | 1 mg/mL |
Shelf Life: | : | 1 year |
Blood Group Specificity: | : | Non-specific |
Preferred Sugar Specificity: | : | Sialic Acid |
Inhibiting or Eluting Sugar: | : | Lactose > β-Galactose |
Fluorescence: | : | Orange |
Divalent Ions: | : | None Required |
Excitation: | : | 550 nm |
Emission: | : | 570 nm |
Mitogenic Activity: | : | Yes |
Lyophilized or Liquid | : | Liquid |
Storage Temperature: | : | 2-8°C |
Hazardous Shipping: | : | Non-hazardous |
Applications
References
- Inoue K, Wada J, Eguchi J, Nakatsuka A, Teshigawara S, Murakami K, et al. (2013) Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray. PLoS ONE 8(10): e77118. https://doi.org/10.1371/journal.pone.0077118