Sambucus nigra Lectin (SNA/EBL I+II) - Colloidal Gold

Sambucus nigra Lectin (SNA/EBL I+II) - Colloidal Gold
Sambucus nigra (Elderberry) Lectin (SNA/EBL I+II) - Colloidal Gold
Product image 1Sambucus nigra Lectin (SNA/EBL I+II) - Colloidal Gold
Product image 2Sambucus nigra (Elderberry) Lectin (SNA/EBL I+II) - Colloidal Gold

Price: Regular price $142.89

SKU#: 21511175-1

Product Description

Affinity purified Sambucus nigra lectin (EBL I+II, also known as SNA) is is composed of two ricin-related lectins. EBL I+II is a tetrameric glycoprotein with a recognition of the Neu5Ac(α2-6)Gal or GalNAc linkage in glycoconjugates.EBL I+II is not blood group specific but it does agglutinate type A erythrocytes more strongly than either B or O cells. Trypsin treated erythrocytes react better than untreated cells. Neuraminidase treated erythrocytes react with this lectin due to the presence of terminal galactose resides. SNA has a broad specificity for both α- and β-linked galactose, as well as unrelated sugars such as fucose. It has been determined that the lectin does not bind to glycoproteins or glycolipids containing only terminal α(2,3)-linked sialic acid residues.EBL I is composed of an A-chain with enzymatic activity and a B-chain with carbohydrate-binding activity. EBL II consists only of carbohydrate-binding B-chains. EBL I+II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems independent from the catalytic activity of the A-chain, but depends on the carbohydrate binding B-chain.Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Sambucus nigra Lectin (SNA/EBL I+II). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: SNA/EBL I+II
Material Source:: Elderberry
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Sialic Acid
Inhibiting or Eluting Sugar:: Lactose > β-Galactose
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

  • Inoue K, Wada J, Eguchi J, Nakatsuka A, Teshigawara S, Murakami K, et al. (2013) Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray. PLoS ONE 8(10): e77118. https://doi.org/10.1371/journal.pone.0077118
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