Psophocarpus tetragonolobus Lectin (PTL/PTA II) - Colloidal Gold

Psophocarpus tetragonolobus Lectin (PTL/PTA II) - Colloidal Gold
Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA II) - Colloidal Gold
Product image 1Psophocarpus tetragonolobus Lectin (PTL/PTA II) - Colloidal Gold
Product image 2Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA II) - Colloidal Gold

Price: Regular price $153.32

SKU#: 21511145-1

Product Description

Psophocarpus tetragonolobus lectin (PTL II, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by fractionation. The seed contains two lectins in which PTL II is the acidic of the two. This lectin agglutinates trypsinized and desialized erythrocytes of all three blood group types (A, B, and O). Nonpolar interactions play an important role in stabilization of the lectin-sugar complex. Projection of α1,3 linked Gal/GalNAc leads to steric hindrance and prevents the binding of blood group A and B reactive sugars.The unique specificity of PTL II makes it to be useful in isolation and characterization of terminally monofucosylated H-reactive oligosaccharides from those that are difucosylated or internally fucosylated.

Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Psophocarpus tetragonolobus Lectin (PTL/PTA II). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: PTL/PTA II
Material Source:: Winged bean
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine, Galactose
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Divalent Ions:: Ca++, Mn++
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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