Psophocarpus tetragonolobus Lectin (PTL/PTA I) - Colloidal Gold

Psophocarpus tetragonolobus Lectin (PTL/PTA I) - Colloidal Gold
Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA I) - Colloidal Gold
Product image 1Psophocarpus tetragonolobus Lectin (PTL/PTA I) - Colloidal Gold
Product image 2Psophocarpus tetragonolobus (Winged bean) Lectin (PTL/PTA I) - Colloidal Gold

Price: Regular price $186.70

SKU#: 21510961-1

Product Description

Psophocarpus tetragonolobus lectin (PTL I, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by affinity chromatography. The seed contains two lectins in which PTL I is the basic of the two. PTL I is a dimeric glycoprotein with specificity to GalNAc, with eluting sugar GalNAc. This lectin agglutinates trypsinized rabbit and human erythrocytes (type A and B) but not trypsinized human type O erythrocytes. PTL I recognize blood group A-substance derived oligosaccharides such as trisaccharide GalNAcα1-3Galβ1-4Glc.

Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Psophocarpus tetragonolobus Lectin (PTL/PTA I). Colloidal gold labeled lectins can be viewed via electron microscopy.

Technical Specifications

Abbreviation: PTL/PTA I
Material Source:: Winged bean
Conjugate:: Colloidal Gold
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine, Galactose
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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