Maackia amurensis Lectin (MAA/MAL II) is affinty puried hemagglutinating lectin and can be used to detect O-linked glycans containing the trisaccharide Siaα2-3Galß1-3GalNAc. This lectin does not agglutinate one blood type more than another and agglutination can be inhibited by lactose at high concentrations. Although MAA I and MAA II are similar in size and structure, this lectin exhibits more hemagglutinating ability than MAA I.
Cy3 can be used to viualize the binding pattern of Maackia amurensis Lectin (MAA/MAL II) in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.