Affinity purified Hippeastrum hybrid lectin (HHL) is consists of four subunits of identical size. It binds only to (α-1,3) and (α-1,6) linked mannosyl units of α-mannose residues. HHL reacts not only with terminal but also with internal α-D-mannosyl residues. Lectin appears to possess extended binding sites complementary to at least three 1,6-linked α-mannosyl. HHL also binds to some yeast galactomannans.
Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Hippeastrum hybrid Lectin (HHL) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.