Griffonia simplicifolia Lectin (GSL I) - TRITC (Rhodamine)
Price: Regular price $152.15
Product Description
Griffonia simplicifolia lectin (GSL I) is affinity purified from a woody climbing shrub native to Africa. GSL I is composed of two types of subunits A and B that combine to form tetrameric structures, resulting in five isolectins. This lectin has affinity for terminal α-D-galactosyl residues and terminal N-acetyl-α-D-galactosaminyl residues. The A-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the B-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. This lectin shows specificity for blood group B greater than blood group A1.GSL I has been reported to bind several glycoproteins including laminin. Since some α-D-galactosyl residues are expressed in endothelial cells of mouse tissues, GSL I can present a highly specific surface of glycosylation pattern in these cells.Griffonia simplicifolia Lectin (GSL I) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled GSL I can be used for cellular imaging applications.
Technical Specifications
Abbreviation | : | GSL I |
Material Source: | : | Bandeiraea |
Conjugate: | : | Tetramethylrhodamine (TRITC) |
Concentration: | : | 2 mg/mL |
Shelf Life: | : | 1 year |
Blood Group Specificity: | : | Non-specific |
Preferred Sugar Specificity: | : | α-Galactose, α-N-Acetylgalactosamine |
Inhibiting or Eluting Sugar: | : | Galactose, N-Acetylgalactosamine |
Fluorescence: | : | Orange |
Divalent Ions: | : | Ca++, Mn++ |
Excitation: | : | 545-555 nm |
Emission: | : | 570-580 nm |
Mitogenic Activity: | : | No |
Lyophilized or Liquid | : | Liquid |
Storage Temperature: | : | -20°C |
Hazardous Shipping: | : | Non-hazardous |