Dolichos biflorus Lectin (DBA) - HRP (Horseradish Peroxidase)

Dolichos biflorus Lectin (DBA) - HRP (Horseradish Peroxidase)
Dolichos biflorus (Horse gram) Lectin (DBA) - HRP (Horseradish Peroxidase)
Product image 1Dolichos biflorus Lectin (DBA) - HRP (Horseradish Peroxidase)
Product image 2Dolichos biflorus (Horse gram) Lectin (DBA) - HRP (Horseradish Peroxidase)

Price: Regular price $203.39

SKU#: 21510045-1

Product Description

Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with a molecular weight of about 111,000 and consists of 4 subunits of equal size. This lectin has an isoelectric point between pH 4.6 and pH 5.5. It has a carbohydrate specificity toward α-N-acetylgalactosamine and elutes with the sugar GalNAc.

DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition and for detecting blood typing. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells, and is used to distinguish these blood types in routine serology applications, and has potential applications in examining tumors which affect the levels of blood group substances.

Reconstitute with sterile buffer. Calcium, manganese, magnesium, and zinc ions are required for binding.

Horseradish peroxidase (HRP) is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or release of light as a byproduct of the reaction. It is most commonly used for blotting, immunoassays and immunohistochemistry methods. Dolichos biflorus Lectin (DBA) is conjugated to HRP at an appropriate ratio to provide optimal staining characteristics.

Technical Specifications

Abbreviation: DBA
Material Source:: Horse gram
Conjugate:: Horseradish Peroxidase (HRP)
Concentration:: 5 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Divalent Ions:: Ca++, Mn++, Mg++, Zn++
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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