Concanavalin A Lectin (Con A) - Colloidal Gold
Price: Regular price $155.30
Product Description
Affinity purified concanavalin A (Con A) is composed of two identical subunits of 237 amino acid residues (MW: 26,000 without any cystine residues). While above pH 7 it is predominantly tetrameric, at pH 4.5 – 5.6, Con A binds two metal ions per monomer: transition metals, Mn2+ and Ca2+. Both ions are required for binding with optimum activity at a pH 7.0. Con A binds with non-reducing α-D-glucose, α-D-mannose and α-methyl-D-glucopyranoside acts as a competitive inhibitor. Con A does not have a blood group specificity. It exhibits mitogenic activity with lymphocytes and cancer cells. While lymphocytes and cancer aggregate by Con A; normal white cells do not. Normal cells react to Con A after proteolytic treatment which suggests that trypsinization causes clustering of the reactive glycan residues on the membrane. Con A interacts with cell types include locust muscle fibers, adipocytes, and rat liver plasma membrane components. This lectin induces endoreduplication in mammalian cells and it reacts with E. coli, Dictyostelium discoideum and Bacillus lipopolysaccharides. Immobilized Con A has been used in affinity chromatography purifications of a wide variety of glycoproteins and cellular structures.Colloidal gold can be used to visualize localization of specific saccharide moieties and examine the distribution of plasma membrane glycoproteins based on the binding pattern of Concanavalin A (Jackbean) Lectin (Con A). Colloidal gold labeled lectins can be viewed via electron microscopy.
Technical Specifications
Abbreviation | : | Con A |
Material Source: | : | Jackbean |
Conjugate: | : | Colloidal Gold |
Concentration: | : | 1 mg/mL |
Purity: | : | High Purity Grade |
Shelf Life: | : | 1 year |
Blood Group Specificity: | : | Non-specific |
Preferred Sugar Specificity: | : | α-Mannose, α-Glucose |
Inhibiting or Eluting Sugar: | : | α-Methylmannoside + α-Methylglucoside |
Divalent Ions: | : | Ca++, Mn++ |
Excitation: | : | 550 nm |
Emission: | : | 570 nm |
Mitogenic Activity: | : | Yes |
Lyophilized or Liquid | : | Liquid |
Storage Temperature: | : | 2-8°C |
Hazardous Shipping: | : | Non-hazardous |