Jacalin lectin (AIA) is purified by affinity chromatography and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the Î±-chain. The proteinâ€™s structure explains itâ€™s carbohydrate-binding specificity of T-antigen disaccharide GalÎ²1,3GalNAc. Potent inhibitors of jacalin include D-galactose, Î²-Met-Gal and 2-deoxy-Î±-D-galactose.Artocarpus integrifolia (Jacalin) Lectin (AIA)Â is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled AIAÂ can be used for cellular imaging applications.