Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein's structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galβ1,3GalNAc. Potent inhibitors of jacalin include D-galactose, β-Met-Gal and 2-deoxy-α-D-galactose. AIA is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled AIA can be used for cellular imaging applications. This product comes in a stabilized liquid form.
- Source: Artocarpus integrifolia (Jackfruit)
- ActivityLess than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes.
- Protein Concentration (Based on OD 280): 1 mg purified AIA - TRITC / 1 mL buffer
- Carbohydrate Specificity: Galß3GalNAc
- Inhibitory Carbohydrate: Galactose or Melibiose
- Divalent Ions Required: None
- Conjugation: TRITC (Rhodamine)
- Fluorescence: Red-Orange fluorescent
- Excitation: Maximum at 532-546 nm
- Emission: Maximum at 569 nm
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use. Clarify by centrifugation, if needed.