Agaricus bisporus Lectin (ABA/ABL) - AP (Alkaline Phosphatase)

Agaricus bisporus Lectin (ABA/ABL) - AP (Alkaline Phosphatase)

Price: Regular price $159.00

SKU#: 21510704-1

Product Description

Agaricus bisporus agglutinin (ABA) is an affinity-purified tetramer composed of two to four isolectins. ABA has two distinct carbohydrate binding sites, one for galactose-ß-1,3-N-acetylgalactosamine and another for galactose-ß-1,3-N-acetylglucosamine. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent proteins.

Alkaline phosphatase (AP) is conjugated to affinity purified Agaricus bisporus Lectin (ABA/ABL) to show the binding of ABA/ABL in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: ABA/ABL
Material Source:: Mushroom
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galactoseβ1-3N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: Fetuin
Divalent Ions:: None Required
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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