Agaricus bisporus Lectin (ABA/ABL) - AP (Alkaline Phosphatase)
Price: Regular price $171.64
Product Description
Agaricus bisporus agglutinin (ABA) is an affinity-purified tetramer composed of two to four isolectins. ABA has two distinct carbohydrate binding sites, one for galactose-ß-1,3-N-acetylgalactosamine and another for galactose-ß-1,3-N-acetylglucosamine. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent proteins.
Alkaline phosphatase (AP) is conjugated to affinity purified Agaricus bisporus Lectin (ABA/ABL) to show the binding of ABA/ABL in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.
Technical Specifications
| Abbreviation | : | ABA/ABL |
| Material Source: | : | Mushroom |
| Conjugate: | : | Alkaline Phosphatase (AP) |
| Concentration: | : | 1 mg/mL |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galactoseβ1-3N-Acetylgalactosamine |
| Inhibiting or Eluting Sugar: | : | Fetuin |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
References
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7) Perduca, Massimiliano; Destefanis, Laura; Bovi, Michele; Galliano, Monica; Munari, Francesca; Assfalg, Michael; Ferrari, Fabio; Monaco, Hugo L; Capaldi, Stefano; . Structure and properties of the oyster mushroom (Pleurotus ostreatus) lectin\nGlycobiology 2020, (), . https://academic.oup.com/glycob/article-abstract/30/8/550/5716261
8) Cejas, Romina B; Lorenz, Virginia; Garay, Yohana C; Irazoqui, Fernando J; . Biosynthesis of ON-acetylgalactosamine glycans in the human cell nucleus\nJournal of Biological Chemistry 2019, 294(9), 2997-3011. https://www.jbc.org/content/294/9/2997
9) Cejasa, Romina B; Garaya, Yohana C; de la Fuente, Sofia; Lardone, Ricardo D; Irazoqui, Fernando J; . Core 1 O-GalNAc glycosylation in the human cell nucleus\nBiological Chemistry 2019, 1(just-accepted), . https://www.degruyter.com/view/journals/bchm/401/9/article-p1041.xml
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