Artocarpus integrifolia Lectin (AIA) - Cy3

Artocarpus integrifolia Lectin (AIA) - Cy3

Price: Regular price $192.46

SKU#: 21761090-1

Product Description

Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography (SKU: 21510001) and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein's structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal and 2-deoxy-α-D-galactose. Cy3 can be used to viualize the binding pattern of Artocarpus integrifolia Lectin in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.


  • Source: Artocarpus integrifolia (Jackfruit)
  • Activity: Less than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes.
  • Protein Concentration (Based on OD 280): 1 mg purified AIA- Cy3 / 1 mL buffer
  • Carbohydrate Specificity: Galß1-3GalNAc
  • Inhibitory Carbohydrate: Galactose or Melibiose
  • Divalent ions required: None
  • Mitogenic Activity: Yes
  • Conjugation: Cy3
  • Excitation: Maximum at 550 nm
  • Emission: Maximum at 570 nm
  • Fluorescence: Orange

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.

Technical Specifications

Abbreviation: AIA
Material Source:: Jacalin
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Appearance Form:: Liquid
Appearance Color:: Redish
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galβ3GalNAc
Inhibiting or Eluting Sugar:: Galactose or Melibiose
Fluorescence:: Orange
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous



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