Arachis hypogaea agglutinin (PNA) is purified by affinity chromatography (SKU: 21510000) and is consisting of four subunits of approximately 27,000 each. This lectin has an isoelectric point between pH 5.5 and pH 6.5. It has a carbohydrate specificity for Galβ3GalNAc with an eluting sugar of galactose. Galactose and lactose are poor inhibitors while disaccharide Gal β(1->3)GalNAc and desialylated human erythrocyte N-substance (T-antigen which is present in many M and N blood groups) are potent inhibitors of PNA. PNA may be used for clinical determination of T-polyagglutinability of erythrocytes and probing cell membranes of normal and malignant cells. This lectin can be of use in characterization of tumor-specific antigens on the surface of malignant cells. It can be employed in the fractionation of stem cells in mice for use in bone marrow transplantation across histocompatibility barriers.
Affinity purified PNA is conjugated to cyanine 3 (Cy3) fluorescent dye. Cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media and are brighter in a non-polar environment than in an aqueous medium. Advantages of plastic over aqueous mounting media are brightness, contrast, and longevity of the fluorescence. Advantage of Cy3 conjugates are bright and photostable in the aqueous environment as well as in non-polar media. This product comes in a stabilized liquid form.
• Source: Arachis hypogaea (Peanut)
• Activity: Less than 1 µg/ml will agglutinate human erythrocytes neuraminidase treatment of the cells.
• Carbohydrate Specificity: Terminal ß-Galactose.
• Inhibitory Carbohydrate: Lactose > ß-Galactose.
• Mitogenic Activity: No
• Divalent Ions Required: Ca2+, Mg2+
• Conjugation: Cy3
• Excitation: Maximum at 550 nm
• Emission: Maximum at 570 nm
• Fluorescence: Orange
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.