Wisteria floribunda Lectin (WFA/WFL) - FITC (Fluorescein)

Wisteria floribunda Lectin (WFA/WFL) - FITC (Fluorescein)

Price: Regular price $190.43

SKU#: 21761065-1

Product Description

Wisteria floribunda agglutinin or lectin (WFA/WFL) is composed of four subunits. This lectin is not blood group specific although it does react stronger with human type A1 cells over A2, B, or O cells. The presence of a sialic acid residue may cause a lack of WFA reactivity. WFA contains a strong hemagglutinin activity and is a potent mitogen, both strongly inhibited by GalNAc, although mitogenic activity is also inhibited by N,N'-diacetylchitobiose. Affinity purified WFA/WFL (SKU: 21510012) is labeled with FITC (fluorescein isothiocyanate) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications. This product comes in a stabilized liquid form.


  • Source: Wisteria Floribunda (Japanese Wisteria)
  • Activity: Less than 4 mg/ml will agglutinate human type O erythrocytes. Less than 1 mg/ml will agglutinate neuraminidase treated erythrocytes.
  • Protein Concentration (Based on OD 280): 2 mg purified WFA - FITC / 1 mL buffer
  • Carbohydrate Specificity: N-Acetylgalactosamine
  • Inhibitory Carbohydrate: Chitin hydrolysateor 500 mM N-Acetylglucosamine with acidor salt
  • Mitogenic Activity: Yes
  • Conjugation: FITC
  • Excitation: Maximum at 495-500 nm
  • Emission: Maximum at 514-521 nm

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8 °C for short term use. Clarify by centrifugation, if needed.

Technical Specifications

Abbreviation: WFA/WFL
Material Source:: Japanese wisteria
Conjugate:: FITC (Fluorescein)
Concentration:: 2 mg/mL
Appearance Form:: liquid
Appearance Color:: Light-yellow
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine, Acetic Acid
Fluorescence:: Green
Divalent Ions:: None Required
Excitation:: 495 nm
Emission:: 515 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous



Close (esc)

10% Discount!

Sign up for our mailing list below and you will receive 10% off your first order of $500.00 or more.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.


Shopping Cart

Your cart is currently empty.
Shop now