Ulex europaeus Lectin (UEA II) - AP (Alkaline Phosphatase)

Ulex europaeus Lectin (UEA II) - AP (Alkaline Phosphatase)

Price: Regular price $208.94

SKU#: 21511194-1

Product Description

Affinity purified Ulex europaeus agglutinin (UEA II) is isolated from gorse seeds and separated from crude extract. This lectin is a glycoprotein that consists of four 24,000 Da monomer subunits that require Ca2+ for binding to its ligands through carbohydrate recognition domain. It is specific for di-N-acetylchitobiose, an oligomer of GlcNAc. The monosaccharide GlcNAc is not an inhibitor of the lectin activity. UEA II has no mitogenic activity and agglutinates type O erythrocytes with greater interest than type A3 erythrocytes. Addition of EDTA eliminates agglutinating activity. Agglutinating activity can be recovered with the addition of calcium.UEA II is a potent inhibitor of the lectin complement pathway following endothelial oxidative stress. The molecular mechanism of UEA II-induced inhibition of the lectin complement pathway appears to be through competition for a common binding site on the endothelial cell and not at the level of mannose-binding lectin (MBL) inhibition. See other UEA II conjugates.Alkaline phosphatase (AP) is conjugated to Ulex europaeus Lectin (UEA II) to show the binding of UEA II in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: UEA II
Material Source:: Gorse
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Oligomers of β(1,4) - linked N-Acetylglucosamine
Inhibiting or Eluting Sugar:: GlcNAc β(1,4) GlcNAc
Divalent Ions:: Ca++, Mn++, Zn++
Lyophilized or Liquid: Liquid
Storage Temperature:: -20°C
Hazardous Shipping:: Non-hazardous

Applications

References

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