Affinity purified Tulip sp. Lectin (TL) lectin is a tetrameric protein composed of four identical subunits which are not held together by disulfide bonds. TL is not blood group specific, but it does agglutinate type O erythrocytes more readily than either A or B cells. It has a carbohydrate specificity of N-acetylgalactosamine, galactose and fucose, and elutes with fetuin or thyroglobulin.
Alkaline phosphatase (AP) is conjugated to Tulip sp. Lectin (TL) to show the binding of TL in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.