Grade: Proteomics Grade
Total Protein Cell Lysis Buffer is a non-ionic detergent lysis buffer that enables efficient solubilization of the plasma and intracellular membranes, breaks weak intermolecular bonds, and solubilizes most proteins. Total Protein Cell Lysis Buffer reduces protein denaturation, protein complex disruption and loss of enzymatic activity since it contains less detergent than a typical RIPA lysis buffer.
Non-ionic detergent reduces denaturation
How to Use
Resuspend cells in Total Protein Cell Lysis Buffer and vortex vigorously. Incubate the cell suspension at 4°C with shaking for 30 minutes. Vortex vigorously for 30 seconds and pellet cellular debris. The supernatant contains the total protein lysate.
pH @25°C: 7.2 - 7.8
Protease (P/F): NONE
Titration (mM): 8 – 12