Styphnolobium japonicum Lectin (SJA)- Cy3

Styphnolobium japonicum Lectin (SJA)- Cy3
Styphnolobium japonicum (Japanese Pagoda tree) Lectin (SJA)- Cy3
Product image 1Styphnolobium japonicum Lectin (SJA)- Cy3
Product image 2Styphnolobium japonicum (Japanese Pagoda tree) Lectin (SJA)- Cy3

Price: Regular price $192.96

SKU#: 21761173-1

Product Description

Styphnolobium japonicum agglutinin (SJA) is affinity purified from Japanese pagoda tree seeds. It consists of two subunits. The subunits can be separated into two subfractions, a D-galactose/N-acetyl-D-galactosamine specific lectin (B-SJA-I) and a D-mannose/D-glucose specific lectin (B-SJA-II). SJA has an isoelectric point between pH 4.9 and pH 5.6 and a carbohydrate specificity towards βGalNAc. This lectin elutes with the sugar GalNAc.

SJA agglutinates all blood group types but has greater affinity for A erythrocytes than of B types, than of O (-SA) types. Binding experiments of the lectin with frozen sections of human kidneys shows specific binding to the endothelia in specimens from blood groups B or AB, thus indicating a D-galactose/N-acetyl-D-galactosamine receptor specificity. SJA lacks mitogenic and immune-suppressive activity.

Cy3 can be used to visualize the binding pattern of Styphnolobium japonicum Lectin (SJA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: SJA
Material Source:: Japanese Pagoda tree
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Appearance Form:: Liquid
Appearance Color:: Orange
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: β-N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: N-Acetylgalactosamine
Fluorescence:: Orange
Divalent Ions:: Ca++, Mn++
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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References

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