Sambucus nigra Lectin (SNA/EBL II) - Cy3

Sambucus nigra Lectin (SNA/EBL II) - Cy3
Sambucus nigra (Elderberry) Lectin (SNA/EBL II) - Cy3
Product image 1Sambucus nigra Lectin (SNA/EBL II) - Cy3
Product image 2Sambucus nigra (Elderberry) Lectin (SNA/EBL II) - Cy3

Price: Regular price $155.41

SKU#: 21511170-1

Product Description

Sambucus nigra lectin (EBL II/SNA II) isaffinity purified from elderberry bark. This lectin is a glycoprotein containing 7.8% carbohydrate and is not blood group specific. It is composed of two subunits and has a molecular weight of 140,000. EBL II has a specificity for carbohydrates containing terminal N-acetylgalactosamine and galactose residues.

The blood group A-active trisaccharide containing L-fucosyl group linked α1-2 to galactose is 10-fold lesser as an inhibitor than the parent oligosaccharide (GalNAc α1-3Gal), which suggests steric hindrance to binding by the α-L-fucosyl group and explains the failure of the lectin to exhibit blood group A specificity.

EBL II induces caspase-dependent apoptosis at low concentrations (nM) order, leading to typical symptoms of cell death in sensitive cells. This effect seems dependent on the carbohydrate binding B-chain.

Cy3 can be used to visualize the binding pattern of Sambucus nigra Lectin (SNA/EBL II) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: SNA/EBL II
Material Source:: Elderberry
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: N-Acetylgalactosamine > Galactose
Inhibiting or Eluting Sugar:: Lactose > β-Galactose
Fluorescence:: Orange
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

  • Inoue K, Wada J, Eguchi J, Nakatsuka A, Teshigawara S, Murakami K, et al. (2013) Urinary Fetuin-A Is a Novel Marker for Diabetic Nephropathy in Type 2 Diabetes Identified by Lectin Microarray. PLoS ONE 8(10): e77118. https://doi.org/10.1371/journal.pone.0077118
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