Psophocarpus tetragonolobus Lectin (PTL/PTA I) - Texas Red
Price: Regular price $232.79
Product Description
Psophocarpus tetragonolobus lectin (PTL I, psophocarpin B1, a storage protein with chymotrypsin inhibitory activity), is isolated from winged bean seeds and purified by affinity chromatography. The seed contains two lectins in which PTL I is the basic of the two. PTL I is a dimeric glycoprotein with specificity to GalNAc, with eluting sugar GalNAc. This lectin agglutinates trypsinized rabbit and human erythrocytes (type A and B) but not trypsinized human type O erythrocytes. PTL I recognize blood group A-substance derived oligosaccharides such as trisaccharide GalNAcα1-3Galβ1-4Glc. Texas Red is a red-fluorescent dye and when bound to Psophocarpus tetragonolobus Lectin (PTL/PTA I) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.
Technical Specifications
Abbreviation | : | PTL/PTA I |
Material Source: | : | Winged bean |
Conjugate: | : | Texas Red |
Concentration: | : | 2 mg/mL |
Purity: | : | High Purity Grade |
Shelf Life: | : | 1 year |
Blood Group Specificity: | : | Non-specific |
Preferred Sugar Specificity: | : | N-Acetylgalactosamine, Galactose |
Inhibiting or Eluting Sugar: | : | N-Acetylgalactosamine |
Fluorescence: | : | Red |
Divalent Ions: | : | None Required |
Excitation: | : | 595 nm |
Emission: | : | 615 nm |
Mitogenic Activity: | : | No |
Lyophilized or Liquid | : | Liquid |
Storage Temperature: | : | -20°C |
Hazardous Shipping: | : | Non-hazardous |