Phaseolus vulgaris Lectin (PHA-E) - Separopore® 4B

Phaseolus vulgaris Lectin (PHA-E) - Separopore® 4B
Phaseolus vulgaris (Kidney bean) Lectin (PHA-E) - Separoporeëå 4B
Product image 1Phaseolus vulgaris Lectin (PHA-E) - Separopore® 4B
Product image 2Phaseolus vulgaris (Kidney bean) Lectin (PHA-E) - Separoporeëå 4B

Price: Regular price $113.69

SKU#: 30330025-1

Product Description

Affinity purified Phaseolus vulgaris lectin (PHA-E) is tetrameric glycoprotein and responsible for the erythroagglutinating properties of the PHA fraction. It has a carbohydrate specificity towards oligosaccharides and elutes with bovine thyroglobulin or acetic acid. PHA-E will bind to both human erythrocytes and lymphocytes, with a specificity towards blood group A (-SA). There are five times more PHA-E receptors on normal human lymphocytes than there are on erythrocytes. The crystal structure of a ligand-free PHA-E has a typical legume lectin fold characterized by two anti-parallel β-sheets and two short α-helices, and contains one GlcNAc residue of the N-linked glycan. Asparagine linked erythrocytes glycopeptide is an inhibitor of PHA-E induced agglutination and mitogenicity, and becomes inactive if treated with β-galactosidase. PHA-E binds di-galactosylated and bisected N-glycan. This lectin is widely used as a biochemical tool for detecting bisecting GlcNAc- and Gal-bearing glycoproteins.

Affinity purified Phaseolus vulgaris Lectin (PHA-E) is immobilized to Separopore® 4B following CNBr coupling. The final product is stabilized in a buffer to retain maximum activity. The gel can be packed in 1 mL or 5mL.

Technical Specifications

Abbreviation: PHA-E
Material Source:: Red kidney bean seeds
Conjugate:: Separopore® 4B
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galß4GlcNAcα2Manα6 (GlcNAcß4) (GlcNAcß4Manα3) Manß4
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

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