Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separopore® matrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.
Imminodiacetate immobilized matrix charged with Ni metal ions.
Chelating group coupling method: epoxy activation
Metal ion capacity: > 20µmol Ni2+/ml gel