Narcissus pseudonarcissus Lectin (NPL/NPA) - Cy3

Narcissus pseudonarcissus Lectin (NPL/NPA) - Cy3

Price: Regular price $158.69

SKU#: 21761126-1

Product Description

Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward α-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar α-methylmannoside.Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.Cy3 can be used to visualize the binding pattern of Narcissus pseudonarcissus Lectin (NPL/NPA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: NPL/NPA
Material Source:: Lent lily
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Molecular Weight:: N/A
Molecular Formula:: N/A
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose
Inhibiting or Eluting Sugar:: α-Methylmannoside
Fluorescence:: Orange
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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