Narcissus pseudonarcissus Lectin (NPL/NPA) - Biotinylated
Price: Regular price $190.72
Product Description
Narcissus pseudonarcissus lectin or agglutinin (NPL/NPA) is affinity purified composed of four subunits. It agglutinates rabbit erythrocytes but is non-reactive with human erythrocytes. NPA has an isoelectric point between pH 4.2 and pH 4.6 with a specific binding activity toward α-D-mannose, although studies have suggested that the purified lectin may have greater affinity for internal mannosyl residues than for nonreducing terminal units. A buffer washed column elutes the sugar α-methylmannoside.Human studies have found NPA to be nearly non-mitogenic, but it has been reported through an in vitro study that mitogenicity of NPA is age-related, with lymphoproliferative response of peripheral blood mononuclear cells (PBMC) in umbilical cord blood has a sevenfold increase than adult blood. NPA has also been found to be mitogenic for PBMC from lambs and not adult sheep.Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated Narcissus pseudonarcissus Lectin (NPL/NPA) has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugate. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.
Technical Specifications
Abbreviation | : | NPL/NPA |
Material Source: | : | Lent lily |
Conjugate: | : | Biotinyl N- Hydroxysuccinimide Ester (Biotin) |
Concentration: | : | 2 mg/mL |
Purity: | : | High Purity Grade |
Shelf Life: | : | 1 year |
Blood Group Specificity: | : | Non-specific |
Preferred Sugar Specificity: | : | α-Mannose |
Inhibiting or Eluting Sugar: | : | α-Methylmannoside |
Divalent Ions: | : | None Required |
Mitogenic Activity: | : | No |
Lyophilized or Liquid | : | Liquid |
Storage Temperature: | : | -20°C |
Hazardous Shipping: | : | Non-hazardous |