Maackia amurensis Lectin (MAA/MAL II) is an affinity purified, hemagglutinating lectin and can be used to detect O-linked glycans containing the trisaccharide Siaα2-3Galß1-3GalNAc. This lectin does not agglutinate one blood type more than another and agglutination can be inhibited by lactose at high concentrations. Although MAL I and MAL II are similar in size and structure, this lectin exhibits more hemagglutinating ability than MAL I.
This lectin is used in a plethora of research. It has been exhibited that MAA inhibits oral squamous cell carcinoma growth and motility by decreasing activity of the JAK-STAT, TGFβ-SMAD, and Wnt-βCTN signaling pathways. MAA has been used to study Turkey adenovirus 3 activity, leaving evidence that the virus has attachment receptors that are both α2,3-linked and α2,6-linked sialic acids. As a sialic acid binding lectin, MAA has been used to observe content of sialic acid in seminoma testicular tissue, revealing that sialic acids may have roles in seminoma development by promoting invasiveness, as well as cross-talk between cancer-cells and the surrounding stroma and vessels. In novel SARS-CoV-2 research, it has been shown that MAA targets the ACE2 receptor, thus decreasing glycosylation and expression. As a result, the spike protein binding is suppressed, and inflammatory mediators have decreased expression. MAA II was shown to inhibit bluetongue virus infection and growth in sheep cells, showing the key functionality of sialic acid as a receptor for viral entry for the virus that affects domestic animals and has a high economic impact. In cholangiocarcinoma research, MAA II positive staining to sialylated glycans revealed elevated levels of glycans in the carcinomic cells over the hyperplastic/dysplastic and normal bile ducts. The same study used the lectin results to suggest that sialylation is involved in 5-fluorouracil resistance development. This product comes in a lyophilized form.
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- Source: Maackia amurensis (Amur maackia)
- Carbohydrate Specificity: Siaα2-3Galß1-3GalNAc
- Inhibitory Carbohydrate: Lactose
- Divalent Ions Required: None
- Mitogenic Activity: Yes
Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles.