Lens culinaris Lectin (LCA/LCH) - Cy3

Lens culinaris Lectin (LCA/LCH) - Cy3
Lens culinaris (Lentil) Lectin (LCA/LCH) - Cy3
Product image 1Lens culinaris Lectin (LCA/LCH) - Cy3
Product image 2Lens culinaris (Lentil) Lectin (LCA/LCH) - Cy3

Price: Regular price $153.32

SKU#: 21761114-1

Product Description

Affinity purified Lens culinaris lectin (LCA) is composed of four subunits that consists of an α-chain (MW: 5.7 kDa) and β-chain (MW: 17.5 kDa). It has a carbohydrate specificity toward α-D-mannose and α-D-glucose, eluting with the sugar MeαMan+MeαGlc. This lectin is useful in affinity chromatography columns for the separation of glycoconjugates. The two isomers LCA-A and LCA-B agglutinates human red blood cells, although not blood group specific. Activity of LCA is determined by haemagglutination with human blood and agglutinates a 2% suspension of human erythrocytes at a lectin concentration of 8µg/ml. LCA requires calcium and manganese ions for binding. See other LCA conjugates.

Cy3 can be used to viualize the binding pattern of Lens culinaris (Lentil) Lectin (LCA/LCH) in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: LCA/LCH
Material Source:: Lentil
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Molecular Weight:: N/A
Molecular Formula:: N/A
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: α-Mannose, α-Glucose
Inhibiting or Eluting Sugar:: α-Methylmannoside + α-Methylglucoside
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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