Datura stramonium Lectin (DSA/DSL) - FITC (Fluorescein)
Price: Regular price $83.44
Product Description
Datura stramonium agglutinin or lectin (DSA/DSL) is purified by affinity chromatography. Seeds of Datura stramonium contain at least three different carbohydrate-binding proteins, and the most prominent lectin is a dimeric glycoprotein composed of two nonidentical subunits that contains 40% carbohydrate. It binds well to N-acetylglucosamine, oligomers, and branched pentasaccharide, including two N-acetylglucosamine disaccharides linked to mannose (β-1,6) and (β-1,2), which is known to be the most potent inhibitor of agglutination. DSL prefers binding to (GlcNAc)2-4 and elutes with the sugar chitin hydrolysate. DSL binds well in the acidic pH range and its affinity decreases above pH 8.0. This agglutinin has blood group A, B, and O specificity. Studies have shown that DSA can act as a biomarker for neoplastic urotherial cells.
Datura stramonium Lectin (DSA/DSL) is labeled with fluorescein isothiocyanate (FITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. FITC conjugates have been used in a variety of immunohistochemical and flow cytometry applications.
Technical Specifications
| Abbreviation | : | DSA/DSL |
| Material Source: | : | Jimson weed |
| Conjugate: | : | FITC (Fluorescein) |
| Concentration: | : | 5 mg/mL |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | (N-Acetylglucosamine)2-4 |
| Inhibiting or Eluting Sugar: | : | Chitin Hydrolysate |
| Fluorescence: | : | Green |
| Divalent Ions: | : | None Required |
| Excitation: | : | 495 nm |
| Emission: | : | 515 nm |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | 2-8°C |
| Hazardous Shipping: | : | Non-hazardous |
