Datura stramonium Lectin (DSA/DSL) - Cy3

Datura stramonium Lectin (DSA/DSL) - Cy3
Datura stramonium (Jimson weed) Lectin (DSA/DSL) - Cy3
Product image 1Datura stramonium Lectin (DSA/DSL) - Cy3
Product image 2Datura stramonium (Jimson weed) Lectin (DSA/DSL) - Cy3

Price: Regular price $153.32

SKU#: 21761100-1

Product Description

Datura stramonium agglutinin or lectin (DSA/DSL) is purified by affinity chromatography. Seeds of Datura stramonium contain at least three different carbohydrate-binding proteins, and the most prominent lectin is a dimeric glycoprotein composed of two nonidentical subunits that contains 40% carbohydrate. It binds well to N-acetylglucosamine, oligomers, and branched pentasaccharide, including two N-acetylglucosamine disaccharides linked to mannose (β-1,6) and (β-1,2), which is known to be the most potent inhibitor of agglutination. DSL prefers binding to (GlcNAc)2-4 and elutes with the sugar chitin hydrolysate. DSL binds well in the acidic pH range and its affinity decreases above pH 8.0. This agglutinin has blood group A, B, and O specificity. Studies have shown that DSA can act as a biomarker for neoplastic urotherial cells.

Cy3 can be used to viualize the binding pattern of Datura stramonium Lectin (DSA/DSL) in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: DSA/DSL
Material Source:: Jimson weed
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: (N-Acetylglucosamine)2-4
Inhibiting or Eluting Sugar:: Chitin Hydrolysate
Fluorescence:: Orange
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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