Bauhinia purpurea Lectin (BPL/BPA) - Cy3

Bauhinia purpurea Lectin (BPL/BPA) - Cy3
Bauhinia purpurea (Camel's foot tree) Lectin (BPL/BPA) - Cy3
Product image 1Bauhinia purpurea Lectin (BPL/BPA) - Cy3
Product image 2Bauhinia purpurea (Camel's foot tree) Lectin (BPL/BPA) - Cy3

Price: Regular price $153.32

SKU#: 21761094-1

Product Description

Bauhinia purpurea agglutinin or lectin (BPA/BPL) is a tetrameric lectin with a molecular weight of 195,000. Binding appears to be highest for glycoconjugates containing galactosyl (β-1,3)N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates.

Although binding specificity is similar to that of peanut agglutinin, tissue staining patterns of these two lectins are distinct. Makela's group 2 sugars, particularly N-acetyl-D-galactosamine, are potent inhibitors. The native protein appears to be stable in detergent solution.

Cy3 can be used to viualize the binding pattern of Bauhinia purpurea Lectin (BPL/BPA) in cellular imaging and flow cytometry. Cy3 is more photostable than many other flourophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling.

Technical Specifications

Abbreviation: BPL/BPA
Material Source:: Camel's foot tree
Conjugate:: Cyanine 3 (Cy3)
Concentration:: 1 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galβ3GalNAc
Inhibiting or Eluting Sugar:: Lactose
Fluorescence:: Orange
Divalent Ions:: None Required
Excitation:: 550 nm
Emission:: 570 nm
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

Close (esc)

10% Discount!

Sign up for our mailing list below and you will receive 10% off your first order of $500.00 or more.

Age verification

By clicking enter you are verifying that you are old enough to consume alcohol.

Search

Shopping Cart

Your cart is currently empty.
Shop now