Bauhinia purpurea Lectin (BPL/BPA) - Biotinylated

Bauhinia purpurea Lectin (BPL/BPA) - Biotinylated

Price: Regular price $166.15

SKU#: 21510030-1

Product Description

Affinity purified Bauhinia purpurea agglutinin or lectin (BPA/BPL) (SKU: 21510028) is a tetrameric lectin with a molecular weight of 120 kDa. Binding is generally highest for glycoconjugates containing galactosyl (ß-1,3) N-acetylgalactosamine structures but oligosaccharides with a terminal α-linked N-acetylgalactosamine can also bind to BPL. BPA is lactose-specific and elutes with the sugar lactose. It has specificity for blood groups A, B, O (-SA). Treatment of erythrocytes with neuraminidase or trypsin will increase the agglutination reaction, indicating that the receptor is masked by terminal carbohydrates. Makela's group 2 sugars, particularly N-acetyl-D-galactosamine, are potent inhibitors. Although the binding specificity of BPA is similar to that of peanut agglutinin, the tissue staining patterns of these two lectins are distinct. Biotin is a small molecule involved in a wide range of metabolic processes. This ligand forms a complex with Avidin and Streptavidin, resulting in the strongest non-covalent protein-ligand interaction known. Biotinylated BPL/BPA has an appropriate amount of biotin bound to provide optimum detection characteristics when using an Avidin-HRP or Streptavidin-HRP conjugates. Biotinylated lectins allow for more sensitive detection in ELISA and Western-blotting applications.

Specifications:

  • Source: Bauhinia purpurea (Camel's foot tree)
  • Carbohydrate Specificity: Galβ3GalNAc
  • Inhibitory Carbohydrate: Lactose
  • Divalent ions required: None
  • Conjugation: Biotin

Storage and Stability:

Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use.

Technical Specifications

Abbreviation: BPL/BPA
Material Source:: Camel's foot tree
Conjugate:: Biotinyl N- Hydroxysuccinimide Ester (Biotin)
Concentration:: 2 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galβ3GalNAc
Inhibiting or Eluting Sugar:: Lactose
Divalent Ions:: None Required
Mitogenic Activity:: Yes
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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