Artocarpus integrifolia Lectin (AIA) - Texas Red
Price: Regular price $185.27
Product Description
Artocarpus integrifolia Lectin (AIA) is purified by affinity chromatography and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the α-chain. The protein’s structure explains its carbohydrate-binding specificity of T-antigen disaccharide Galß1,3GalNAc. Potent inhibitors of jacalin include D-galactose, ß-Met-Gal and 2-deoxy-α-D-galactose. Affinity purified AIA is labeled with Texas Red dye and comes in a stabilized liquid form.
Texas Red is a red-fluorescent dye that, when conjugated to Artocarpus integrifolia (Jacalin) Lectin (AIA), can reveal the binding pattern of this lectin in cellular imaging applications. The emission spectra of Texas Red have minimal overlap with those of FITC, making this combination suitable for dual-labeling experiments. Compared to other dyes like fluorescein, rhodamine dyes such as Texas Red exhibit greater photostability and reduced sensitivity to pH changes.
Recommended Usage: Recommended dilutions of 0.5 µg / ml in 1XPBS. Less than 0.5 µg / ml will agglutinate neuraminidase treated erythrocytes.
Technical Specifications
| Abbreviation | : | AIA |
| Material Source: | : | Jackfruit Seed |
| Conjugate: | : | Texas Red |
| Concentration: | : | 2 mg/mL |
| Molecular Weight: | : | 53 kDa |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galβ3GalNAc |
| Inhibiting or Eluting Sugar: | : | Galactose or Melibiose |
| Fluorescence: | : | Red |
| Divalent Ions: | : | None Required |
| Excitation: | : | 595 nm |
| Emission: | : | 615 nm |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunofluorescence. Immunohistochemistry. Immunocytochemistry. Cell selection. Glycobiology.
References
- Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (<i>Phaseolus vulgaris</i>) Seeds. Polymers (Basel). 2019 May 2;11(5):785.
- S F S Mohamad et al Response surface optimization of the forward extraction of jacalin from jackfruit seeds using AOT/isooctane reverse micellar system. 2020 IOP Conf. Ser.Mater. Sci. Eng. 716 012019.
- Ahmed H, Chatterjee BP. Further characterization and immunochemical studies on the carbohydrate specificity of jackfruit (Artocarpus integrifolia) lectin. J Biol Chem. 1989 Jun 5;264(16):9365-72.
- Wilkinson R, Neville S. Jacalin: its binding reactivity with immunoglobulin A from various mammalian species. Vet Immunol Immunopathol. 1988 Mar;18(2):195-8.
- T. VIJAYAKUMAR* and J. A. FORRESTER. Purification and Physico-chemical Properties of Lectins from the Jack Fruit (,4rtocarpus iutegrifolia). May 30, 1985; accepted December 9, 1985.
