Jacalin lectin (AIA) is purified by affinity chromatography and is tetrameric two-chain structure consisting of four identical protomers with an isoelectric point of pH 7.8. The lectin has specificity for blood group types O (+SA) and T. Jacalin is considered a galactose-specific lectin and elutes with galactose or melibiose. A post-translational proteolytic modification of Jacalin gives the lectin a novel carbohydrate-binding site involving the N terminus of the Î±-chain. The proteinâ€™s structure explains itâ€™s carbohydrate-binding specificity of T-antigen disaccharide GalÎ²1,3GalNAc. Potent inhibitors of jacalin include D-galactose, Î²-Met-Gal and 2-deoxy-Î±-D-galactose.Affinity purified Artocarpus integrifolia Lectin (AIA) is immobilized to Separopore® 4B following CNBr coupling. The final product is stabilized in a buffer to retain maximum activity. The gel can be packed in 1 mL or 5mL.