Allium sativum Lectin (ASA) - TRITC (Rhodamine)

Allium sativum Lectin (ASA) - TRITC (Rhodamine)
Allium sativum (Garlic) Lectin (ASA) - TRITC (Rhodamine)
Product image 1Allium sativum Lectin (ASA) - TRITC (Rhodamine)
Product image 2Allium sativum (Garlic) Lectin (ASA) - TRITC (Rhodamine)

Price: Regular price $166.15

SKU#: 21761184-1

Product Description

Allium sativum lectin (ASA) is isolated from garlic and purified with affinity chromatography. It is a dimer of two subunits. ASA binds to a number of α1-2-linked mannose residues. The lectin recognizes internal mannose and binds to the core pentasaccharide of N-linked glycans. In addition, the removal of sialic acids enhances binding activity. ASA has shown antiproliferative and apoptosis-inducing activity, making it a relative subject in cancer research.

Purified ASA is labeled with Tetramethylrhodamine isothiocyanate (TRITC) and comes in a stabilized liquid form. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. It has an excitation maximum at 546 nm and an emission maximum at 569 nm. Conjugated form of TRITC is used for cellular imaging applications.

Specifications:
• Source: Allium sativum Lectin (Garlic) ASA
• Activity: Agglutinates rabbit but not human erythrocytes
• Protein Concentration (Based on OD 280): 1 mg purified ASA TRITC/1 mL buffer
• Conjugation: TRITC (Rhodamine)
• Carbohydrate Specificity: Mannose
• Inhibitory Carbohydrate: α(1,3) linked mannosyl units
• Fluorescence: Red-Orange fluorescent
• Excitation: Maximum at 545-55 nm
• Emission: Maximum at 570-580 nm

Storage and Stability:
Store frozen at -20°C in amber vials or covered with foil in appropriate aliquot sizes. Avoid freeze thaw cycles. Can be stored at 2-8°C for short term use. Clarify by centrifugation, if needed.

Technical Specifications

Abbreviation: ASA
Material Source:: Garlic
Conjugate:: Tetramethylrhodamine (TRITC)
Concentration:: 2 mg/mL
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Mannose
Inhibiting or Eluting Sugar:: α(1,3)-Linked Mannosyl Units
Fluorescence:: Orange
Divalent Ions:: None Required
Excitation:: 545-555 nm
Emission:: 570-580 nm
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

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