Agaricus bisporus Lectin (ABA/ABL) - TRITC (Rhodamine)
Price: Regular price $332.40
Product Description
Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose-1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent protein.
Agaricus bisporus Lectin (ABA/ABL) is labeled with tetramethylrhodamine isothiocyanate (TRITC) and has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. TRITC is a bright orange or red-fluorescent dye with excitation ideally suited to the 532-nm laser line. TRITC labeled ABA/ABL can be used for cellular imaging, flowcytometry and other application applications.
Recommended Usage: 1XPBS pH 7.4 can be used to dilute the stocks. Recommended dilution range from 0.5-10 ug/ml. 5-10 µg/ml will agglutinate type A or B cells. 2 µg/ml will agglutinate type O cells. Less than 0.5 µg/ml will agglutinate neuraminidase treated erythrocytes.
Technical Specifications
| Abbreviation | : | ABA/ABL |
| Material Source: | : | Mushroom |
| Conjugate: | : | Tetramethylrhodamine (TRITC) |
| Concentration: | : | 2 mg/mL |
| Molecular Weight: | : | 64 kDa |
| Appearance Form: | : | Liquid |
| Appearance Color: | : | Pink to purple |
| Purity: | : | High purity grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galactoseβ1-3N-Acetylgalactosamine |
| Inhibiting or Eluting Sugar: | : | Fetuin |
| Fluorescence: | : | Orange |
| Divalent Ions: | : | None Required |
| Excitation: | : | 545-555 nm |
| Emission: | : | 570-580 nm |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunocytochemistry, Immunohistochemistry, Immunofluorescence, Glycobiology.
References
1: Santos AO, Abrantes-Coutinho VE, Morais S, Oliveira TMBF. Agaricus bisporus</i> Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor. Biosensors (Basel). 2023 Feb 3;13(2):224.
2.Badr, Mohamed. “Characterization of White Button Mushroom and Its Biomedical Applications.” Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, 2022.
