Agaricus bisporus Lectin (ABA/ABL) - Texas Red
Price: Regular price $332.40
Product Description
Agaricus bisporus Lectin (ABA/ABL) is isolated from edible mushroom and purified by using GalNAc Sepharose 4B. This Lectin is composed of four subunits with slightly different amino acid sequences and glycosylation specificity. ABA/ABL Lectin has high affinity for galactose-1,3-N-acetylgalactosamine (Gal-1,3-GalNAc; also known as T-antigen disaccharide) and galactose-1,3-N acetylglucosamine, but does not bind to monosaccharides. ABA is a potent mitogen for T cells and binds to serum protein IgA. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent protein.
Texas Red is a red-fluorescent dye bound to Agaricus bisporus Lectin (ABA/ABL) can show the binding pattern of this lectin in cellular imaging applications. There is very little overlap between the emission spectra of Texas Red and FITC making this combination ideal for dual-labeling experiments. Rhodamine dyes, such as Texas Red, are more photostable and less sensitive to pH change when compared to other dyes such as fluorescein.
Immunofluorescence, Immunohistochemistry, Cell typing, Glycobiology
Technical Specifications
| Abbreviation | : | ABA/ABL |
| Material Source: | : | Mushroom |
| Conjugate: | : | Texas Red |
| Concentration: | : | 2 mg/mL |
| Molecular Weight: | : | 64 kDa |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 1 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | Galactoseβ1-3N-Acetylgalactosamine |
| Inhibiting or Eluting Sugar: | : | Fetuin |
| Fluorescence: | : | Red |
| Divalent Ions: | : | None Required |
| Excitation: | : | 595 nm |
| Emission: | : | 615 nm |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Cell typing, Diagnostic tool, Glycobiology
References
1: Santos AO, Abrantes-Coutinho VE, Morais S, Oliveira TMBF. Agaricus bisporus</i> Wild Mushroom Extract as Lectin Source for Engineering a Lactose Photoelectrochemical Biosensor. Biosensors (Basel). 2023 Feb 3;13(2):224.
2.Badr, Mohamed. “Characterization of White Button Mushroom and Its Biomedical Applications.” Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, 2022.
