Agaricus bisporus Lectin (ABA/ABL) - AP (Alkaline Phosphatase)

Agaricus bisporus Lectin (ABA/ABL) - AP (Alkaline Phosphatase)

Price: Regular price $171.64

SKU#: 21510704-1

Product Description

Agaricus bisporus agglutinin (ABA) is an affinity-purified tetramer composed of two to four isolectins. ABA has two distinct carbohydrate binding sites, one for galactose-ß-1,3-N-acetylgalactosamine and another for galactose-ß-1,3-N-acetylglucosamine. Agaricus bisporus has an anti-proliferative effect on cancerous cells. ABA can be internalized by clathrin-coated vesicles after binding to surface glycoproteins, thus making ABA an inhibitor of its nuclear import of signal-dependent proteins.
Alkaline phosphatase (AP) is conjugated to affinity purified Agaricus bisporus Lectin (ABA/ABL) to show the binding of ABA/ABL in many applications including Western blotting and ELISA. Alkaline phosphatase is a large protein (140 kDa) that catalyzes the hydrolysis of phosphate groups from a substrate resulting in a colored or fluorescent product. The optimal enzymatic activity of this protein is between pH 8 and 10, and its reaction rate remains linear, improving sensitivity over time.

Technical Specifications

Abbreviation: ABA/ABL
Material Source:: Mushroom
Conjugate:: Alkaline Phosphatase (AP)
Concentration:: 1 mg/mL
Purity:: High Purity Grade
Shelf Life:: 1 year
Blood Group Specificity:: Non-specific
Preferred Sugar Specificity:: Galactoseβ1-3N-Acetylgalactosamine
Inhibiting or Eluting Sugar:: Fetuin
Divalent Ions:: None Required
Mitogenic Activity:: No
Lyophilized or Liquid: Liquid
Storage Temperature:: 2-8°C
Hazardous Shipping:: Non-hazardous

Applications

References

1) Valenti, Maria Teresa; Marchetto, Giulia; Perduca, Massimiliano; Tiso, Natascia; Mottes, Monica; Carbonare, Luca Dalle; . BEL β-Trefoil Reduces the Migration Ability of RUNX2 Expressing Melanoma Cells in Xenotransplanted Zebrafish\nMolecules 2020, 25(6), 1270. https://www.mdpi.com/1420-3049/25/6/1270


2) Petrović, Jovana; Glamočlija, Jasmina; Ilić-Tomić, Tatjana; Soković, Marina; Robajac, Dragana; Nedić, Olgica; Pavić, Aleksandar; . Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma\nInternational Journal of Biological Macromolecules 2020, (), . https://www.sciencedirect.com/science/article/abs/pii/S0141813019381814


3) Das, Banani; De, Biplab; Chetree, Rishiraj; Mandal, Subhash C; . Medicinal Aspect of Mushrooms: A View Point\nHerbal Medicine in India 2020, (), 509-532. https://link.springer.com/chapter/10.1007%2F978-981-13-7248-3_31


4) Singh, Ram Sarup; Walia, Amandeep Kaur; Kennedy, John F; . Mushroom lectins in biomedical research and development\nInternational journal of biological macromolecules 2020, 151(), 1340-1350. https://www.sciencedirect.com/science/article/abs/pii/S0141813019360982


5) Găman, Amelia Maria; Egbuna, Chukwuebuka; Găman, Mihnea-Alexandru; . Natural bioactive lead compounds effective against haematological malignancies\nPhytochemicals as Lead Compounds for New Drug Discovery 2020, (), 95-115. https://doi.org/10.1016/B978-0-12-817890-4.00006-8


6) Chachadi, Vishwanath B; Pujari, Radha; Shastry, Padma; Swamy, Bale M; Inamdar, Shashikala R; . Sclerotium rolfsii lectin induces opposite effects on normal PBMCs and leukemic Molt-4 cells by recognising TF antigen and its variants as receptors\nGlycoconjugate Journal 2020, (), 01-Nov. https://link.springer.com/article/10.1007/s10719-019-09905-y


7) Perduca, Massimiliano; Destefanis, Laura; Bovi, Michele; Galliano, Monica; Munari, Francesca; Assfalg, Michael; Ferrari, Fabio; Monaco, Hugo L; Capaldi, Stefano; . Structure and properties of the oyster mushroom (Pleurotus ostreatus) lectin\nGlycobiology 2020, (), . https://academic.oup.com/glycob/article-abstract/30/8/550/5716261


8) Cejas, Romina B; Lorenz, Virginia; Garay, Yohana C; Irazoqui, Fernando J; . Biosynthesis of ON-acetylgalactosamine glycans in the human cell nucleus\nJournal of Biological Chemistry 2019, 294(9), 2997-3011. https://www.jbc.org/content/294/9/2997


9) Cejasa, Romina B; Garaya, Yohana C; de la Fuente, Sofia; Lardone, Ricardo D; Irazoqui, Fernando J; . Core 1 O-GalNAc glycosylation in the human cell nucleus\nBiological Chemistry 2019, 1(just-accepted), . https://www.degruyter.com/view/journals/bchm/401/9/article-p1041.xml


10) Bogoeva, Vanya; Petrova, Lidiya; Bouckaert, Julie; Yordanova, Anna; Ivanov, Ivan; Vanderesse, Régis; Frochot, Céline; . Dual function of lectins—new perspectives in targeted photodynamic therapy\nJournal of Porphyrins and Phthalocyanines 2019, (), 01-Oct. https://www.worldscientific.com/doi/abs/10.1142/S1088424619300209


11) Sun, Yanan; Zhang, Min; Fang, Zhongxiang; . Efficient physical extraction of active constituents from edible fungi and their potential bioactivities: A review\nTrends in Food Science & Technology 2019, (), . https://www.sciencedirect.com/science/article/abs/pii/S0924224418301018


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