BCIP

BCIP

Price: Regular price $66.18

SKU#: 21530050-1

Product Description

BCIP
Also known as 5-Bromo-4-Chloro-3-Indolyl Phosphate. A widely used chromogenic substrate for alkaline phosphatase in enzyme-linked assay systems. BCIP may be used alone, or may act in a coupled reaction with NBT. Upon cleavage of the phosphate group from the molecule, BCIP forms a bright blue, insoluble product. This product then reacts to form a chromogen homodimer. This reaction may be used to amplify the signal by catalyzing the reduction of a tetrazolium salt (usually NBT) to form an intenselycolored, insoluble formazan chromogen. For best results, protect from light and allow to reach ambient temperature before use. 

Technical Information:

  • Ultra Pure Grade
  • Purity (enzymatic): >99.0%
  • Purity (UV): >99.0%
  • Inhibitors (enzymatic): <0.1%
  • Ref: Unger, et al., Am. J. Surg. Pathol., 10(1): 1-8, 1986.
  • Technical Specifications

    Molecular Weight:: 433.63
    Molecular Formula:: C15H15BrCIN2O4P
    Appearance Form:: Powder
    Appearance Color:: White
    Purity:: >97%
    Storage Temperature:: 2-8°C
    Hazardous Shipping:: Non-hazardous

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