Oncorhynchus keta Lectin (CSL3) - Cy3
Price: Regular price $699.00
Product Description
Affinity purified Oncorhynchus keta Lectin (CSL3) (SKU: 21510016) was isolated from the eggs of chum salmon, a species of anadromous fish. CSL3 is a homodimer that has four outward-oriented domains, thus acquiring pseudo-tetrameric structure. CSL3 has shown agglutination towards rabbit, sheep, and human type A, B, and O erythrocytes. Each 20 kDa subunit is composed of two carbohydrate-binding sites containing domains. It has been shown that CSL3 evokes apoptosis when bound to Gb3 as a cell receptor, pointing to its potential applications in cancer. Cy3 can be used to visualize the binding pattern of CSL3 in cellular imaging and flow cytometry. Cy3 is more photostable than many other fluorophores and can be seen with TRITC filter sets. It is commonly combined with green-fluorescent dyes for dual-labeling. Cyanine dyes are better able to withstand the harsh dehydration and embedding conditions required for mounting sections in non-polar plastic media and are brighter in a non-polar environment than in an aqueous medium. Advantages of plastic over aqueous mounting media include quality of brightness, contrast, and longevity of fluorescence. This product comes in a stabilized liquid form.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg/ml in 1XPBS. Less than 16 μg/ml of CSL3 will agglutinate rabbit/porcine erythrocytes.
Technical Specifications
| Abbreviation | : | CSL3 |
| Material Source: | : | Chum Salmon Egg |
| Conjugate: | : | Cyanine 3 (Cy3) |
| Concentration: | : | 1 mg/mL |
| Molecular Weight: | : | 23 kDa |
| Appearance Form: | : | Liquid |
| Appearance Color: | : | Orange |
| Purity: | : | High Grade Purity |
| Shelf Life: | : | 2 year |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | L-Rhamnose |
| Inhibiting or Eluting Sugar: | : | L-Rhamnose >> Melibiose, Raffinose |
| Fluorescence: | : | Orange |
| Divalent Ions: | : | None Required |
| Excitation: | : | 550 nm |
| Emission: | : | 570 nm |
| Lyophilized or Liquid | : | Liquid |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Blotting, Bioconjugation, Diagnostic tool for cancer, Cell typing/sorting, Glycobiology.
References
- Shirai, T., Watanabe, Y., Lee, M.-sub, Ogawa, T., & Muramoto, K. (2009). Structure of Rhamnose-binding Lectin CSL3: Unique Pseudo-tetrameric Architecture of a Pattern Recognition Protein. Journal of Molecular Biology, 391(2), 390–403.
- Watanabe, Y., Tateno, H., Nakamura-Tsuruta, S., Kominami, J., Hirabayashi, J., Nakamura, O., Watanabe, T., Kamiya, H., Naganuma, T., Ogawa, T., Naudé, R. J., & Muramoto, K. (2009). The function of rhamnose-binding lectin in innate immunity by restricted binding to Gb3. Developmental & Comparative Immunology, 33(2), 187–197.
