Lycopersicon esculentum Lectin (LEL/LEA) - Pure
Price: Regular price $204.72
Product Description
Lycopersicon esculentum lectin (LEL/LEA) is Isolated and affinity-purified from tomatoes, has carbohydrate specificity for GlcNAc. Composed of a single stable subunit, LEL serves as a valuable marker for tracking vasculature in rodents and neuroscience research. With an unusually high carbohydrate content of about 50 percent arabinose and galactose, LEL non-specifically agglutinates human erythrocytes of blood types A, B, O, and AB. Protease-treated erythrocytes show slightly increased sensitivity to agglutination by this lectin.
Recommended Usage: Recommended dilutions of 0.5 10 μg/ml in 1XPBS pH 7.4. 50-60 µg/ml will agglutinate type O human erythrocytes. 5-10 µg/ml will agglutinate neuraminidase treated cells.
Technical Specifications
| Abbreviation | : | LEL/LEA |
| Material Source: | : | Tomato |
| Conjugate: | : | None |
| Concentration: | : | 2 mg, 5 mg |
| Molecular Weight: | : | 71 kDa |
| Appearance Form: | : | Lyophilized Powder |
| Appearance Color: | : | Off white - brown |
| Purity: | : | Affinity purified |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | Non-specific |
| Preferred Sugar Specificity: | : | ß (1,4)-linked-N-Acetylglucosamine |
| Inhibiting or Eluting Sugar: | : | Chitin Hydrolysate |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Vascular Imaging, Blood vessels mapping in CNS, Bioconjugations, Cellular analysis, Diagnostic tools, Glycobiology.
References
- Naito Y, Minamihara T, Ando A, Marutani T, Oguri S, Nagata Y. Domain construction of cherry-tomato lectin: relation to newly found 42-kDa protein. Biosci Biotechnol Biochem. 2001 Jan;65(1):86-93.
- Keiko SAITO, Hiromi MAGI, Kei'ichi BABA, Irwin J. GOLDSTEIN and Akira MISAKI Oyo. Purification, Properties and Carbohydrate-Binding Specificity of Cherry Tomato (Lycopersion esculentum var. Cherry) Lectin Toshitsu Kagaku (J. Appl. Glycosci.), Vol. 43, No. 3, p. 331-345 (1996).
