Maclura pomifera Lectin (MPL/MPA) - Pure
Price: Regular price $225.42
Product Description
Maclura pomifera agglutinin or lectin (MPA/MPL) is tetrameric plant protein purified by affinity chromatography. MPA has a molecular weight of about 44,000 and consists of four subunits. It has an isoelectric point between pH 4.8 and pH 5.3, and blood group specificity towards A, B, and O (-SA). This lectin has a high affinity for tumor-associated T-antigen disaccharide (Galβ1,3GalNAcα) and many O-linked glycopeptide structures. The structure of Galβ1,3GalNAcα occurs on the surface of tumor cells as a mucin-associated antigenic marker, termed as T-antigen. It is also the central element in O-linked glycopeptide structures on other mammalian glycoproteins.
Recommended Usage: Recommended dilutions of 0.5 – 10 μg/ml in 1XPBS. : Less than 5 mg/ml will agglutinate type O human erythrocytes. Less than 0.1 mg/ml will agglutinate neuraminidase treated cells.
Technical Specifications
| Abbreviation | : | MPL/MPA |
| Material Source: | : | Osage orange Seed |
| Conjugate: | : | None |
| Concentration: | : | 5 mg, 10 mg |
| Molecular Weight: | : | 40 kDa |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A, B, O (-SA) |
| Preferred Sugar Specificity: | : | GalNAc, Melibiose |
| Inhibiting or Eluting Sugar: | : | Methyl α-D-Galactopyranoside |
| Divalent Ions: | : | None Required |
| Mitogenic Activity: | : | Yes |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Bioconjugations, Blotting, Cell typing, Rat lymphoid cell binding, Mitogenic stimulation, Glycobiology.
References
- Bausch JN, Poretz RD. Purification and properties of the hemagglutinin from Maclura pomifera seeds. Biochemistry. 1977 Dec 27;16(26):5790-4.
