Dolichos biflorus Lectin (DBA) - Pure
Price: Regular price $181.13
Product Description
Dolichos biflorus agglutinin (DBA) is isolated from horse gram and purified by affinity chromatography. DBA is a glycoprotein with 4 equal-sized subunits, a molecular weight of about 111 kDa, and an isoelectric point between pH 4.6 and pH 5.5. DBA has been used as a general marker of developing renal collecting ducts, which can then be used to establish secretion status in blood group A individuals by hemagglutination inhibition. DBA is over thousand times more active in agglutinating blood group A1 cells versus A2 cells and is used to distinguish these blood types in routine serology applications. DBA glycosylation in the differentiation of basal squamous epithelial stem cells has been studied due to its cancer applications, revealing a possible relationship between the DBA-binding sites and integrin β1 retention in supra-basal movement of differentiated basal cells. The enhanced DBA-binding of hepatocellular carcinoma cells that were treated with a multikinase inhibitor, sorafenib and the subsequent blocking of the Ras/Raf/mitogen activated signaling pathway exemplifies the novel treatments that can be discovered using DBA lectin blotting. In developmental research, DBA allows for the identification of spermatogonia stem cell markers integrin β1 and PLZF. The sensitive detection of uterine natural killer cell localization using DBA has been useful in placental development and trophoblast invasion research. DBA also has applications for non-cancer diseases. This lectin is known to bind to the Forssman antigen (FA) expressed by acids on the wall of streptococcus pneumoniae and has been used to investigate the enzymatic activity of phosphorylcholine esterase in isolates of the disease.
Recommended Usage: Recommended dilutions of 0.5 – 10 µg/ml in 20mM Tris-HCl pH 7.5, 1 mM CaCl2, 0.5 mM ZnCl2, 0.5 mM MnCl2, 0.5 mM MgCl2, 50 mM NaCl. DBA will agglutinate human type A1 cells by 4 µg/ml. Human type A2 cells will be agglutinated with higher concentrations of DBA (200 µg/ml).
Technical Specifications
| Abbreviation | : | DBA |
| Material Source: | : | Horse gram |
| Conjugate: | : | None |
| Concentration: | : | 5 mg, 10 mg |
| Molecular Weight: | : | 140 kDa |
| Appearance Color: | : | Off white to brown |
| Purity: | : | High Purity Grade |
| Shelf Life: | : | 2 years |
| Blood Group Specificity: | : | A1>>A2 |
| Preferred Sugar Specificity: | : | GalNAc |
| Inhibiting or Eluting Sugar: | : | GalNAc(α1-3)GalNAc |
| Divalent Ions: | : | Ca++, Mn++, Mg++, Zn++ |
| Mitogenic Activity: | : | No |
| Lyophilized or Liquid | : | Lyophilized |
| Storage Temperature: | : | -20°C |
| Hazardous Shipping: | : | Non-hazardous |
Applications
Blood typing, Blood grouping, Tumor marker, Blotting, Immunohistochemistry, Bioconjugation, Glycobiology.
References
- Dvořánková, B., MotlÃk, J., HolÃková, Z., VacÃk, J., & Smetana, K. (2012). Dolichos biflorus agglutinin-binding site expression in basal keratinocytes is associated with cell differentiation. Biology of the Cell, 94(6), 365–373.
- Liu, T., Liu, R., Zhang, S., Guo, K., Zhang, Q., Li, W., & Liu, Y. (2017). Sorafenib induced alteration of protein glycosylation in hepatocellular carcinoma cells. Oncology letters, 14(1), 517–524.
- Valdivia, M., Castañedaâ€Zegarra, S., Lévano, G., Lazo, J., Reyes, J., Bravo, Z., Santiani, A., Mujica, F., RuÃz, J., & Gonzales, G. F. (2019). Spermatogonial stem cells identified by molecular expression of PLZF, integrin β1 and reactivity to Dolichos biflorus agglutinin in alpaca adult testes. Andrologia, 51(6).
- Valdivia, M., Reyes, J., Bravo, Z., Cancho, C., Castañeda, S., Limaymanta, O., Woll, P., Santiani, A., & Gonzales, G. F. (2019). In vitro culture of spermatogonial stem cells isolated from adult alpaca (Vicugna pacos) testes analysed withDolichos biflorusby flow cytometry. Andrologia, 51(6).
- Zhong, T., Xie, X., Zong, T., Yu, X., Ling, Y., & Kuang, H. (2018). Lectin histochemical analysis of uterine natural killer cells in normal, hydatidiform molar and invasive molar pregnancy. Oncology letters, 16(5), 6458–6464.
- Zhou, M. L., Frost, M. R., Xu, Y. C., & Nahm, M. H. (2020). Phosphorylcholine esterase is critical for Dolichos biflorus and Helix pomatia agglutinin binding to pneumococcal teichoic acid. Journal of basic microbiology, 60(10), 905–915.
